According to the World Health
Organization (WHO), malaria killed
an estimated 655,000 people, mostly children, in 2010. Artemisinin is an effective antimalarial drug recommended by the WHO to be used in combination therapies. Artemisinin-based
treatments could prove to be a silver bullet for the malaria scourge affecting
developing areas of the world. There's just one catch: artemisinin is derived
from Artemisia annua (Wormwood), an
herb. Artemisia farming depends on
the weather. Artemisinin may be only a small, small part of the overall plant
mass, meaning that a great deal of resources (water, land, etc) are needed to
produce small amounts of the desired drug. Thus, the current method of artemisinin production is unpredictable and inefficient. Queue, genetic engineering.
Molecular Biology - The Portal to Biotechnology
Suppose a researcher wants a cell to produce a
particular protein—say, Green Fluorescent Protein (GFP). Now that we understand
the central dogma, the researcher's path is pretty straight forward. First, the researcher
would need a copy of the gene, usually from an existing source, like
jellyfish DNA. There are many ways to insert the gene into a cell, and more
ways are being explored. Let's say for now that the researcher puts the GFP
gene on a plasmid (a circular piece
of DNA that is self-replicating in a cell). To cut-and-paste a piece of DNA,
scientists use restriction enzymes,
which are like molecular scissors. They recognize specific sequences of the DNA
alphabet and sever double stranded DNA in predictable ways. DNA ligase is like the glue, that bonds
strands back together.
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